Composite

Part:BBa_K902087:Design

Designed by: Somshukla Chaudhuri   Group: iGEM12_Calgary   (2012-10-25)

moa Operon (MoaABCDE)-J04500


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 539
    Illegal BamHI site found at 665
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 952
    Illegal AgeI site found at 1851
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 603
    Illegal SapI.rc site found at 1964


Design Notes

moaABCDE operon followed by lacI promoter (IPTG-repressible) and a ribosomal binding site (rbs).

Source

E. coli

References